Mad Science – Luciferase, Crispr, mRNA, What Could Go Wrong?
The luciferase gene from the firefly, Photinus pyralis, was used as a reporter of gene expression by light production in transfected plant cells and transgenic plants.
A complementary DNA clone of the firefly luciferase gene under the control of a plant virus promoter (cauliflower mosaic virus 35S RNA promoter) was introduced into plant protoplast cells (Daucus carota) by electroporation and into plants (Nicotiana tabacum) by use of the Agrobacterium tumefaciens tumor-inducing plasmid. Extracts from electroporated cells (24 hours after the introduction of DNA) and from transgenic plants produce light when mixed with the substrates luciferin and adenosine triphosphate.
Light produced by the action of luciferase was also detected in undisrupted leaves or cells in culture from transgenic plants incubated in luciferin and in whole transgenic plants “watered” with luciferin.
Although light was detected in most organs in intact, transgenic plants (leaves, stems, and roots), the pattern of luminescence appeared to reflect both the organ-specific distribution of luciferase and the pathway for uptake of luciferin through the vasculature of the plant.
Firefly Luciferase mRNA (ARCA, 5-moUTP) expresses luciferase protein which is initially extracted from firefly Photinus pyralis. The enzyme catalyzes ATP-dependent D-luciferin oxidation to yield oxyluciferin, a singlet-excited compound that emits light when returning to its ground state. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for gene expression, cell viability and in vivo imaging etc.
Our Firefly Luciferase mRNA is provided at a concentration of 1mg/ml, it is ARCA capped, polyA-tailed and incorporated with modified nucleotides. The capping of ARCA (anti-reverse cap analog) ensures high translation efficacy. Poly (A) tail plays an important role in enhancing the efficiency of translation initiation. The addition of 5-moUTP and poly (A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo.https://www.youtube.com/embed/RNRZchHaKgw?feature=oembed
Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP)
Catalog No. R1005
Better performance of firefly luciferase mRNA modified by ARCA, 5mCTP and ΨUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control.
Magnetofection™ is a novel, simple and highly efficient method to transfect cells in culture. It exploits magnetic force exerted upon gene vectors associated with magnetic particles to draw the vectors towards, possibly even into, the target cells. In this manner, the full vector dose applied gets concentrated on the cells within a few minutes so that 100% of the cells get in contact with a significant vector dose. This has several important consequences:
- Greatly improved transfection rates in terms of percentage of cells transfected compared to standard transfection.
- Up to several thousand fold increased levels of transgene expression compared to standard transfections upon short-term incubation.
- High transfection rates and transgene expression levels are achievable with extremely low vector doses, which allows to save expensive transfection reagents.
- Extremely short process time. A few minutes of incubation of cells with gene vectors are sufficient to generate high transfection efficiency, compared to several hours with standard procedures.
Magnetofection™ Reagents
As the manufacturer of the Magnetofection™ technology, chemicell offers two types of ready-to-use Magnetofection™ reagents.
PolyMAG is a universally applicable magnetic particle preparation for high efficiency nucleic acid delivery. It is mixed in a one-step procedure with the nucleic acid to be transfected and has been used successfully with plasmid DNA, antisense oligonucleotides and siRNAs.
CombiMAG is a magnetic particle preparation designed to be combined with any commercially available transfection reagent such as polycations and lipids and can be associated with plasmid DNA, antisense oligonucleotides, siRNAs or viruses. It allows you to create your own magnetic gene vector based on your favourite transfection reagent.
What can be used for good has reached Frankenstein proportions in pushing mankind into transhuman goals for mad scientists who want to see what happens when they do it. Transhumanism is mingling of man’s seed, turning them into creation of man, controlled by man, soulless, mind controlled robotic things.This is all way above any humans pay grade to understand. The one fact that is clear to understand is MAN IS PLAYING GOD producing man made souless creatures and they have now crossed the line of health and fitness into creation of monsters.
Transhumanism is the globalist RESET future for humanity. This is as the days of Noah and in the end, God will destroy all of it – forever. Like it or not, this is where we are. Behold, this is what they do.
Then Gates says it’s to help the poor? Give me a break! Does the world’s poor really need a Gates super modified transhuman world?
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Tom
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What could go wrong?…with the current gaggle of intellectual retards running the show…not a thing.
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